T7

Part:BBa_K3476011

Designed by: Silvio Salvatore Bonni   Group: iGEM20_MSP-Maastricht   (2020-10-19)


bacterial shRNA_expression_cassette

Allows for the expression of hairpin RNA-sequences. The part can be used in T7-polymerase expressing, RNAseIII free bacterial strains. ShRNA coding DNA-sequences can be cloned in as a dsDNA-oligos using the EcoR1 and BamH1 restriction sites. Multiple cassettes coding different RNAs can be chained together using Gibson assembly. Expression is under IPTG-induction.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 56
    Illegal NheI site found at 116
    Illegal NotI site found at 31
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 56
    Illegal BamHI site found at 62
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 56
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//direction/forward
//function/structures
//plasmid/expression/t7
transcriptional
Parameters
None